Fig. 3.

(A) The fit of a first order rate equation to the disappearance of H₂O₂ from the medium by respiring mitochondria. Note, although only 5 mM malate or 5 mM malate plus 2 µM auranofin are illustrated, similar fits and variation were found for all other conditions. Solid lines are the mean for each condition with SEM indicated by light grey (n=4 for each condition). Dotted lines (r²=0.98 for mean malate and 0.99 for mean malate+auranofin values) represent regressions fitting [H₂O₂]_t=[H₂O₂]₀×e−k_app·pr·t to the mean data values. (B) Extrapolating the inverse relationship between $V_{app}^P$ and k_app to determine k. Data are mean±SEM (n=4–6) based on H₂O₂ efflux (Fig. 2A) and calculated first order rate constant using the consumption of H₂O₂ based on [H₂O₂]_t = [H₂O₂]₀⋅e^{−k_app·pr·t} and [H₂O₂]₀ set at 2000 nM. Black is control, grey is with 2 µM auranofin present. Estimates for k when $V_{app}^P$ is zero are 0.045 and 0.018 under control and auranofin inhibited conditions respectively.