Figure 7.
The Annexin V apoptosis detection kit using flow cytometry determined an increasing number of Paclitaxel (PTX) treated hASCs entered early apoptosis as compared to untreated control hASCs. A. 15.8% of control cells in the early apoptotic phase. B. After 24 hours of Paclitaxel treatment, 18.8% of hASCs were in early apoptosis. C. After 3 days of Paclitaxel treatment, 30.8% of hASCs were in early apoptosis. D. When 1 dose of Paclitaxel was given, followed by medium changes without Paclitaxel for 7 days, 51.8% of hASCs were in early apoptosis. E. After 7 days of continual treatment with Paclitaxel, 64.7% of hASCs were in early apoptosis.