Figure 6.

Furosemide toxicity and JNK activation in HepaRG cells. (A) Differentiated HepaRG cells were treated with increasing concentrations of furosemide (FS) or with PBS vehicle control. Lactate dehydrogenase (LDH) release was measured 24 h later. (B) HepaRG cells were treated with 10 mM FS and LDH release was measured at various time points. (C) HepaRG cells were treated with FS for various time points and JNK activation was measured by western blotting. HepaRG cells were also treated with 200 μM tert-butyl hydroperoxide (tBHP) for 2 h as a positive control. Data are expressed as mean ± SEM for n = 3 experiments. *p < 0.05 compared with control.