Assessment of electron transport chain complex function in PINK1 KO animals. Mitochondrial assessment of the electron transport chain complexes in the cortex and striatum as of 4 month old (A & C, respectively) and 9 month old (B & D, respectively) as assessed by oxygen consumption rate (OCR) and measured by a Seahorse XF24 analyzer. Mitochondrial were treated to subsequent injections of Rotenone, Succinate, Antimycin A and TMPD/Asc while measuring OCR. Statistical significance was assessed by a repeated-measures two-way ANOVA followed by Sidak’s post-hoc test. *: p≤0.05 on Sidak’s post-hoc comparison test. For all experiments, n=3 biological replicates.