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. 2015 Jun;145(6):529–541. doi: 10.1085/jgp.201411349

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© 2015 Kim et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 8. — M₁ muscarinic stimulation induces fast inactivation of Ca_V2.2 current by eliciting transient and reversible translocation of the β2e subunit from membrane to cytosol. (A) Current inhibition of Ca_V2.2 channels by M₁R activation with Oxo-M and current inactivation of Ca_V2.2 channels before Oxo-M and during Oxo-M addition in cells expressing β2a, β3, β2e, or N-del. Current traces for before (a) and during (b) Oxo-M addition were superimposed (inset). (B) Current inactivation with various β subunits was recorded during a 500-ms test pulse at 10 mV. Current traces before (black trace) and during (red trace) Oxo-M applications were scaled to the peak current amplitude (I₀) and were superimposed. Dashed line indicates zero currents. (C) Summary of Ca_V2.2 current inhibition by M₁R stimulation in cells expressing β2a (n = 4), β3 (n = 6), β2e (n = 8), or N-del (n = 9). ***, P < 0.001, compared with β2a and β2e, respectively. (D) Summary of current inactivation before and during Oxo-M application in cells expressing β2a (n = 6), β3 (n = 5), β2e (n = 5), or N-del (n = 5). *, P < 0.05, compared with control. Data are mean ± SEM.