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. 2015 Jun;145(6):555–563. doi: 10.1085/jgp.201511365

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© 2015 Ravera et al.

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Figure 3. — Ranges of dissociation constants for Na⁺ binding by WT NIS. (Top) Dissociation constants for Na⁺ binding by empty WT NIS—K_d,A (red) and K_d,B (green)—as a function of the allowed values for the association constant K_a,A. (Bottom) Enhanced dissociation constant for the second site when the first site is already occupied.