Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 May 25;209(4):595–608. doi: 10.1083/jcb.201409142

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Luo et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 1. — Tkv acts in ECs to restrict germline proliferation. (A) Schematic of a Drosophila germarium. (B and C) Compared with a control (c587ts/+) germarium (B), a tkvⁱ (C; BL40937) germarium contains ectopic spectrosome-containing cells. Vasa (green) is a germ cell marker. (D) Statistical data showing the number of spectrosome-containing cells in control (c587ts/+), tkvⁱ (c587ts;tkvⁱ[BL-40937]), off-target construct rescue (c587ts;UAS-tkv[off-target]), or c587ts;tkvⁱ/UAS-tkv[off-target] germaria. (E–G) A germarium carrying tkv⁸ mutant ECs (lack of GFP signal marked by arrowheads) exhibits more spectrosome-containing cells (E and F), which is rescued by restoring tkv expression (G). (E′) Cartoon model to illustrate the position of mutant EC clones (blue). The genotype of E is c587.UAS-flp; FRT40A.ubiGFP/FRT40A.tkv⁸. The genotype of G is c587.UAS-flp; FRT40A.ubiGFP/FRT40A.tkv⁸;UAS-tkv. (H) In addition to the germline expression (white arrows indicate GSCs and CBs and yellow arrows indicate developing oocytes), tkv mRNA is strongly detected in the ECs (arrowheads). (I) A c587ts;UAS-CD8GFP germarium exhibits strongly colocalized Tkv and GFP staining in ECs (arrows). Bars, 10 µm. ***, P < 0.001.