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. 2015 May 25;209(4):595–608. doi: 10.1083/jcb.201409142

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© 2015 Luo et al.

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Figure 5. — Wnt signaling acts in the ECs to regulate Tkv expression. (A–F) Germaria with compromised Wnt signaling components (Arm [A and B, BL-35004] and Lgs [C, BL-37476]) or with overexpressed TCF^DN (D and E) in ECs exhibit more spectrosome-containing cells and reduced tkv^k16713 reporter expression. (F) Statistical data for spectrosome-containing cells in armⁱ or TCF^DN expressing germarium. (G) A germarium containing ECs mutant for the pygo^s123 allele (generated using the MARCM system and marked with an arrowhead) exhibits ectopic spectrosome-containing cells. Genotype: hs-flp.UAS-CD8.GFP/+;tub-gal4/+;FRT82B.pygo^s123/FRT82B.tub-gal80. (H) A pygoⁱ (NIG-11518R) germarium harbors ectopic Dad-lacZ–positive spectrosome-containing cells. (I) A pygoⁱ (NIG-11518R-1) germarium contains more pMad-positive spectrosome-containing cells. (J) A armⁱ (BL-35004) germarium expresses low levels of tkv transcripts (compared with Fig. 1 H). (K) A lgsⁱ (BL-37476) germarium expresses low levels of Tkv in the ECs (compared with Figure 1 I). (L) One EC mutant for pygo^s123 (arrowhead) exhibits reduced Tkv expression, compared with control EC (arrow) of the same germarium. Genotype: hs-flp;FRT82B.pygo^s123/FRT82B.ubi-GFP. (M–O) Forced expression of Tkv in lgsⁱ (BL-37476) germaria strongly suppresses the formation of ectopic spectrosome-containing cells. ***, P < 0.001. Bars, 10 µm.