Table 1.
Antibodies Used in Western Blot Analysis.
| Specificity | Region | Manufacturer | Catalog No | Species | Vehicle | Working Dilution |
|---|---|---|---|---|---|---|
| Primary Antibodies | ||||||
| TRPV1 | C-terminal | Alomone Labs (Jerusalem, Israel) | ACC-030 | Rabbit | 1% BSA, 0.05% NaN3 in PBS | 1:200 |
| TRPV1 | C-terminal | Abcam (Cambridge, UK) | Ab45759 | Mouse | 1% BSA, 0.05% NaN3 in PBS | 1:200 |
| TRPV1 | C-terminal | Sigma-Aldrich (St Louis, MO) | V2764 | Rabbit | 1% BSA, 0.05% NaN3 in PBS | 1:500 |
| β-actin | N-terminal | Sigma-Aldrich | A2228 | Mouse | 1% non-fat milk in TBS-T | 1:2000 |
| Secondary Antibodies | ||||||
| Rabbit IgG | Cell Signaling Technology (Beverly, MA) | 7074 | 1% non-fat milk in TBS-T | 1:2000 | ||
| Mouse IgG | Sigma-Aldrich | A4416 | 1% non-fat milk in TBS-T | 1:5000 | ||
BSA, bovine serum albumin; PBS, phosphate-buffered saline; TBST, Tris-buffered saline containing 1% Tween-20.