Fig. 1.

Role of IL-17A treatment on lipid metabolism of DCs. A: GO “biological processes” with significant overrepresentation among the genes significantly upregulated in DC-17s (n = 5) versus untreated DCs (n = 4) (P ≤ 0.01 and expression fold change ≥2). Assignment to GO groups was possible for 853 out of 1,184 probe sets overexpressed in DC-17s versus DCs. The fold enrichment for the eight significantly enriched pathways (P < 0.05) is presented. B–E: Total lipids were extracted from DCs of three different donors, either untreated at day 0 (DC) or treated with IL-17A at indicated time points (DC-17). The different lipid classes were separated by thin-layer chromatography, and the amount of phospholipids, cholesterol, triglycerides, and cholesteryl ester was analyzed by GC or by GC/MS in DCs.