Fig. 4.

Functional phenotype of molecules involved in lipid anabolism and lipid uptake in DC-17s. A–D: mRNA expression measured by Affymetrix microarray in untreated DCs at day 0 (n = 4) and DC-17s treated with IL-17A for 12 days (n = 5). Results are means ± SD. Statistical significance was determined with LIMMA.* P < 0.05; ** P < 0.01 for enzymes of fatty acid synthesis (A), scavenger receptors (B), fatty acid transport proteins (C), and LDLR-family members (D). E–F: Flow cytometry analysis of fatty acid uptake in DCs from three donors. Untreated DCs at day 0 (DC) or DC-17s cultured for 5 days with IL-17A were incubated with fluorescently labeled C16 fatty acid (Bodipy-FL-C16) for 30 min at 4°C or 37°C. E: Representative staining for donor 1. F: Shift in the Bodipy-FL-C16 mean fluorescence intensity between 37°C and 4°C for three donors in separate experiments.