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. 2015 Jun;56(6):1134–1144. doi: 10.1194/jlr.M057661

Fig. 1.

Fig. 1.

LPA and S1P are poor inhibitors of ATX activity at physiological concentrations of substrate. A: LPA and S1P potently inhibit ATX activity (ATX at 100 ng/ml concentration) when incubated with 5 μM FS-3 in the fluorogenic assay. However, this inhibition does not occur in the presence of 200 μM LPC in the choline-release assay (ATX at 1,000 ng/ml concentration). B: Increasing the FS-3 concentration decreases the inhibition effect of 1 μM LPA or S1P on ATX activity. Results are mean ± SEM from three independent experiments. *A significant increase (P < 0.05) in activity in the presence of S1P with 15 μM FS-3 compared with 5 μM FS-3. **A significant increase (P < 0.05) in activity in the presence of S1P at 50 μM FS-3 compared with 5 or 15 μM FS-3. #A significant increase (P < 0.05) in activity in the presence of LPA at 50 μM FS-3 compared with 5 or 10 μM FS-3.