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. 2015 Jun;56(6):1134–1144. doi: 10.1194/jlr.M057661

Fig. 4.

Fig. 4.

LPA and S1P inhibit ATX mRNA expression through LPA and S1P receptor-mediated activation of phosphatidylinositol 3-kinase. A: SW-579 cells were incubated in 10% FBS or FBSC in the presence of 5 μM LPA or S1P, or 1 μM of the LPA receptor agonist wls-31 or S1P receptor agonist SEW2871. B: ATX mRNA expression increased in cells incubated in FBS when treated with 1 μM of the LPA receptor antagonist Ki16425 or SIP receptor antagonist VPC23019. These antagonists had no effect on ATX mRNA levels in cells incubated in FBSC alone. C: ATX mRNA expression increased in cells incubated in FBS when treated with phosphatidylinositol 3-kinase inhibitors (10 μM LY294402 or 1 μM wortmannin). These inhibitors had no effect on ATX mRNA levels in cells in FBSC alone. Results are mean ± SEM from three independent experiments. *A significant increase (P < 0.05) in ATX mRNA compared with FBS treatment. #A significant decrease (P < 0.05) in ATX mRNA compared with FBSC treatment.