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. 2015 Jun;56(6):1134–1144. doi: 10.1194/jlr.M057661

Fig. 5.

Fig. 5.

LPC-mediated inhibition of ATX mRNA expression is blocked by ATX inhibitors. A: SW-579 cells were incubated in 10% FBS or FBSC supplemented with 1% BSA in the presence of 5 μM LPA and/or 100 μM LPC with 10 μM of the ATX inhibitors ONO-8430506 (ONO) or S32826. B: SW-579 and MDA-MB-435S cells were incubated for 48 h in medium under the same conditions as (A). Secreted ATX protein was quantified by ELISA and normalized to both volume of conditioned medium and cell lysate protein content. ATX protein from basal medium was subtracted from the total ATX protein measured after incubation. Results are mean ± SEM from three independent experiments. *A significant increase (P < 0.05) compared with FBS treatment. #A significant decrease (P < 0.05) compared with FBSC treatment.