TABLE 2.

Primer sequences used for RT-PCR analyses

Gene	Function	Primer	Sequence
β-actin	Housekeeping	Forward	5′-GGCTGTATTCCCCTCCATCG-3′
		Reverse	5′-CCAGTTGGTAACAATGCCATGT-3′
MGAT2	TG synthesis	Forward	5′-TGGGAGCGCAGGTTACAGA-3′
		Reverse	5′-CAGGATGGCATACAGGACAGA-3′
GPAT3	TG synthesis	Forward	5′-TATCCAAAGAGATGAGTCACCCA-3′
		Reverse	5′-CACAATGGCTTCCAACCCCTT-3′
GPAT1	TG synthesis	Forward	5′-CTGCTTGCCTACCTGAAGACC-3′
		Reverse	5′-GATACGGCGGTATAGGTGCTT-3′
DGAT1	TG synthesis	Forward	5′-TCCGTCCAGGGTGGTAGTG-3′
		Reverse	5′-TGAACAAAGAATCTTGCAGACGA-3′
DGAT2	TG synthesis	Forward	5′-TTCCTGGCATAAGGCCCTATT-3′
		Reverse	5′- AGTCTATGGTGTCTCGGTTGAC-3′
MGL	MG hydrolysis	Forward	5′- CAGAGAGGCCCACCTACTTTT-3′
		Reverse	5′- ATGCGCCCCAAGGTCATATTT-3′
ACC1	Lipogenesis	Forward	5′-AGGTGGTGATAGCCGGTATGT-3′
		Reverse	5′-TGGGTAATCCATAGAGCCCAG-3′
FAS	Lipogenesis	Forward	5′-ATGGGCGGAATGGTCTCTTTC-3′
		Reverse	5′-TGGGGACCTTGTCTTCATCAT-3′
FAAH	Acylethanolamide hydrolysis	Forward	5′-GAGGCTCCCCTCTGGGTTTA-3′
		Reverse	5′-GCCAGGCTATCCACATCCC-3′
CB1	CB receptor	Forward	5′- GGGCACCTTCACGGTTCTG-3′
		Reverse	5′- GTGGAAGTCAACAAAGCTGTAGA-3′
NPY	Orexigenic neuropeptide	Forward	5′-ATGCTAGGTAACAAGCGAATGG-3′
		Reverse	5′-TGTCGCAGAGCGGAGTAGTAT-3′
AgRP	Orexigenic neuropeptide	Forward	5′-ATGCTGACTGCAATGTTGCTG-3′
		Reverse	5′-CAGACTTAGACCTGGGAACTCT-3′