Fig. 2.

The effect of 4F on SMase-induced LDL aggregation at acidic pH. LDL particles (1 mg/ml) were modified with hSMase in the absence or presence of the indicated molar ratios of L-4F to apoB-100 at different pH. The aggregation of the LDL particles was monitored by turbidity (A) and by SEC (B). The sizes of the LDL particles were measured using DLS (C) at the indicated time points. The formation of phosphorylcholine was determined by an Amplex Red assay (D). The results represent means ± SD of triplicate wells and are representative of two independent experiments performed with different LDL preparations.