Fig. 4.

The effect of 4F on SMase-premodified LDL. LDL particles (1.25 mg/ml) were modified with SMase at pH 7.0 for 15 min after which lipolysis was stopped and the premodified LDL particles (1 mg/ml) were incubated with and without L-4F at 10:1 molar ratio of 4F to apoB-100. The aggregation of the LDL particles was monitored by turbidity (A) and by SEC (B). The inset in B shows quantitative analysis of aggregation based on the SEC data. The sizes of the LDL particles were measured using DLS (C) at the indicated time points. The results are representative of two independent experiments performed with different LDL preparations.