Fig. 7.

The 4F peptide prevents SMase-induced conformational changes of apoB-100. LDL particles (2 mg/ml) were incubated with and without L-4F at 10:1 molar ratios of 4F to apoB-100 at pH 7.0 for 30 min, followed by extensive dialysis. Control and 4F-pretreated LDL (1 mg/ml) were modified with bcSMase at pH 7.0 for 15, 30, or 60 min. The samples were diluted to (50 µg/ml) for far UV-CD measurement. The results are representative of two independent experiments performed with different LDL preparations.