Fig. 8.

The 4F peptide decreases the binding of SMase-modified LDL to human aortic proteoglycan. Control and 4F-pretreated LDL particles (1 mg/ml) with molar ratio of L-4F to apoB-100 ranging from 2.5:1 to 20:1 were modified with bcSMase at pH 7.0 for 15 min and aliquots were added to microtiter wells precoated with human aortic proteoglycans. The amounts of proteoglycan-bound LDL were determined by measuring the amounts of cholesterol in the wells. The results represent means ± SD from triplicate wells and are representative of two independent experiments performed with different LDL preparations.