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. 2015 Mar 15;8(3):3420–3428.

Figure 2.

Figure 2

miR-298 targets BAX through interaction with BAX 3’-UTR. A. miR-298 reduces BAX mRNA levels. MGC803 and SGC7901 cells were transfected with miR-298 or control oligonucleotide for 48 h and then subjected to RT-PCR analyses. B. Knockdown of miR-298 induces BAX protein level. MGC803 and SGC7901 cells were transfected with miR-298 and control for 48 h and then examined for immunoblotting of BAX. C. Sequence alignment of the human miR-298 sequences with a region of the BAX 3’-UTR from indicated species. D. Luciferase reporter assay. The indicated cells were transfected with the indicated plasmids and oligonucleotide as well as β-galactosidase. After a 24 h incubation, cells were subjected to a luciferase assay. Luciferase activity was normalized with β-galactosidase. Relative luciferase activities are presented. Data represent three independent experiments in triplicate. **Significant difference from control, P < 0.01.