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Mixed lymphocyte reaction (MLR). T-cell stimulatory capacity of transduced DCs was assess with MLR. DCs obtained from BALB/c (H-2d) mice were treated with HBcAg antigen (DC/HBcAg) or rAds (DC/Ad-C, DC/Ad-S, DC/Ad-C+Ad-S, and DC/Ad-lacZ), and then subjected to allogeneic (A) and autologous (B) MLR involving T cells from C57BL/6 (H-2Kb) mice and BALB/c mice, respectively, at various R/S ratios. After 4 d, T cell proliferation was assessed by the [3H] incorporation assay.
