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Validation of immunohistochemical staining data by real-time RT-PCR assays. The relative transcriptional levels for PKD1 gene was determined by real-time RT-PCR. The real-time RT-PCR log2 values were plotted against the immunohistochemical staining data log2 values. There sult showed a strong positive correlation (R2 = 0.999) between the two techniques.
