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. 2015 Mar 15;8(3):3801–3808.

Figure 4.

Figure 4

MiR-615 suppresses AKT2 expression by directly targeting the AKT2 3’-UTR and altered levels of proteins related to cell proliferation and cell cycle in MDA-MB231 cells. A. Predicted miR-615 target sequence in the 3’-UTR of AKT2 (AKT2-3’-UTR) and positions of three mutated nucleotides in the 3’-UTR of miR-615 (miR-615-mut). B. AKT2 protein expression in MDA-MB231 cells transfected with miR-615 or the miR-615 inhibitor were detected by Western blotting analysis. α-Tubulin served as the loading control. C. Luciferase reporter assay of MDA-MB231 cells transfected with the pGL3-AKT2-3’-UTR reporter and miR-615 or miR-615-in or miR-615-mut or NC with oligonucleotides. D. Real-time PCR analysis of expression of Cyclin D1, p27 and p21 in MDA-MB231 cells. E. Cyclin D1, p27, p21, phosphorylated retinoblastoma (p-Rb) and Rb were measured by western blot in MDA-MB231 cells. α-Tubulin was used to serve as the loading control. *P < 0.05.