Fibrogenic cytokine and chemokine in the skin. Mice were locally treated with either PBS or BLM for 5 days, and APS was administered intravenously immediately after initiating BLM treatment. Skin samples were homogenized, centrifuged, and supernatants measured by ELISA to detect the expression of MCP-1 (A), INF-γ (B), TNF-α (C), and IL-4 (D).