Table 1.
cDNA primer sequences used for reverse transcriptase-polymerase chain reaction to test the expression of HMGB1 and TLR4 gene in asthmatic mice lung tissues
Gene | Forward primer | Reverse primer | Product length |
---|---|---|---|
HMGB1 | CGGATGCTTCTGTCAACT | TCAGCTTGGCAGCTTTCT | 360 bp |
TLR4 | GGTGAGAAATGAGCTGGTA | TCTGCTAAGAAGGCGATA | 313 bp |
GAPDH | CCACTTGAAGGGTGGAGC | TGAAGTCGCAGGAGACAA | 530 bp |