PRV-614/MC4R-GFP double-labeled neurons in the dorsal motor nucleus of the vagus nerve (DMV). Image (A) taken from Toshiko Tsumori (Brain Res 2014) indicates the approximate location from which the images were digitized. Images (B, D) were taken from an animal after injections of PRV-614. Image (B) showed neurons infected with PRV-614, which send transsynaptic projections to the stomach; Image (C) showed MC4R-GFP positive neurons in the DMV; Image (D) showed overlaid images of (B) plus (C). Arrows indicate double-labeled neurons. DMV, dorsal motor nucleus of the vagus nerve; 12, hypoglossal nucleus. Scale bars, 50 μm.