Figure 3.

The effects of Jinlida on AMPK signaling and adiponectin receptor gene expression in skeletal muscle in rats. The mRNA expression levels of adiponectin receptor 1 (ADIPOR1), adiponectin receptor 2 (ADIPOR2), AMP-activated protein kinase (AMPK), Acetyl-CoA carboxylase (ACC) and glucose transporter type 4 (GLUT4) were examined by quantitative real-time-polymerase chain reaction (A-F). The corresponding bands of western blotting were showed (H).The protein levels of P-AMPK, P-ACC, AMPK, ACC and GLUT4 were detected by Western blotting (G-K). We calculated the expression of proteins as [(OD target protein/OD β-actin) experiment group]/[(OD target protein/OD β-actin) control group]. So the standard deviation in ND group was 1. n = 6 per group; *P < 0.05 compared with the HFD group; #P < 0.05 compared with the ND group.