Table 4.
Primer sequences for quantitative polymerase chain reaction
| Gene | Forward primer (5’-3’) bp | Reverse primer (5’-3’) bp | Amplified fragment length |
|---|---|---|---|
| ADIPOR1 | CCGCATCCACACAGAAACT | ACATCCCGAAGACCACCTT | 139 |
| ADIPOR2 | TGTAAGGTGTGGGAAGGTCG | GGAAAGAAGGCATAGGAGGC | 111 |
| PPAR γ | CCACCAACTTCGGAATCAG | GATGTCAAAGGAATGGGAGTG | 68 |
| PPARα | GGTCCGATTCTTCCACTGCT | GGTAACCTGGTCATTCAAGTCC | 115 |
| ACADM | TGACGGAGCAGCAGAAAGAG | TTGATGAGAGGGAACGGGT | 115 |
| NRF1 | AGACACGGTTGCTTCGGAA | CGCACCACATTCTCCAAAG | 148 |
| COX IV | TCGCTGAGATGAACAAGGG | AGTGAAGCCGATGAAGAACA | 74 |
| PGC1α | AAGACCAGGAAATCCGAGC | TTGCCATCCCGTAGTTCAC | 103 |
| CPT1 | CCAAACATCACTGCCCAA | GGAAATAGGCTTCGTCATCC | 100 |
| GLUT4 | ATACTCATTCTCGGACGGTTC | CCCACATACATAGGCACCAA | 74 |
| ACCβ | TGCCCACTTTCTTCTATCACG | ACATAAACCTCCAGGGACGC | 61 |
| AMPKα1 | AATCCAAACACCAAGGCG | TGCTCTACACACTTCTGCCAT | 95 |
| β-actin | TGTGATGGTGGGTATGGGT | AGGATGCCTCTCTTGCTCTG | 69 |
ADIPOR1: Adiponectin receptor 1; ADIPOR2: Adiponectin receptor 2; PPARγ: Peroxisome proliferator-activated receptor gamma; PPARα: Peroxisome proliferator -activated receptor α; ACADM: acyl-CoA dehydrogenase; NRF1: nuclear respiratory factor 1; COX IV: cytochrome c oxidase subunit IV; PGC1α: Peroxisome proliferator-activated receptor gamma, coactivator 1 alpha; CPT1: Carnitine palmitoyltransferase 1; ACC: Acetyl-CoA carboxylase; AMPKα1: Adenosine 5’-monophosphate-activated protein kinase α1; GLUT4: Glucose transporter type 4.