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The pedicel of a longan fruit showing the pre-existing abscission zone (AZ) with a visible sunken line (thick arrow). The inset shows the vertical section around the AZ. The section between the solid lines was sampled for observation of tissue distribution of ROS using fluorescent dye 2′,7′-dichlorofluorescin diacetate (DCFH-DA). Abscission zone, i.e., section between the dashed lines was sampled for observation of the subcellular changes in structure and H2O2 distribution.
