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. 1972 Nov;2(5):395–401. doi: 10.1128/aac.2.5.395

Antiviral Action of Camptothecin1

Susan B Horwitz 1,2, Chung-Ke Chang 1,2, Arthur P Grollman 1,2
PMCID: PMC444326  PMID: 4363789

Abstract

At a concentration of 10 μm, camptothecin inhibited vaccinia deoxyribonucleic acid (DNA) synthesis in HeLa cells. Inhibition of viral DNA synthesis was observed when the drug was added before infection or at 1 or 2 hr after infection. Inhibitory effects of camptothecin on vaccinia DNA synthesis could be reversed, even after exposure to the alkaloid for 2 hr. Viral DNA, isolated from vaccinia-infected, camptothecin-treated cells, displayed an altered sedimentation constant after alkaline sucrose density gradient centrifugation. Incorporation of uridine into vaccinia messenger ribonucleic acid was inhibited by camptothecin, but the activity of ribonucleic acid polymerase, as tested in isolated vaccinia cores, was not affected by the drug. Camptothecin had essentially no effect on replication of poliovirus in HeLa cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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