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. 2015 May 4;112(20):6377–6382. doi: 10.1073/pnas.1418736112

Fig. 5.

Fig. 5.

Mutations in the Dnm1p binding sites of Pex11p disturb peroxisome fission. (A) Fluorescence microscopy analysis of methanol-grown pex11Δ cells (pex11Δ) and pex11Δ cells producing WT Pex11p (WT) or Pex11p L15A/L59A (L15A/L59A). Cells coproduced the peroxisomal membrane marker PMP47-GFP. (Scale bar, 5 μm.) (B) Quantification of peroxisome numbers in pex11Δ cells producing WT or mutant forms of Pex11-His6. All strains coexpressed PMP47-GFP, the readout of which was used to quantify peroxisome numbers. Values represent the mean ± SD of two independent experiments (*P < 0.05). (C) Western blots of cell lysates from pex11Δ cells producing WT or mutant forms of Pex11p, coproducing PMP47-GFP. Blots were probed with antibodies raised against elongation factor (EF) 1α (loading control), GFP, and the His6 tag.