Table 1.
Peptide sequencea | # of residues | Unmodified peptide mass (Da)b | Unmodified peptide Ω (Å2) | Pal-peptide mass (Da)c | Pal-peptide Ω (Å2) | ΔΩ (Å)d | # of Pal group(s) | ΔΩ per Pal group (Å2) |
---|---|---|---|---|---|---|---|---|
CHDFR | 5 | 676.28 | 185.4 | 914.51 | 239.3 | 53.9 | 1 | 53.9 |
MGCDR | 5 | 580.21 | 173.7 | 818.44 | 217.7 | 44.0 | 1 | 44.0 |
MCILK | 5 | 606.32 | 182.1 | 844.55 | 230.2 | 48.2 | 1 | 48.2 |
MACLK | 5 | 564.28 | 179.3 | 802.50 | 218.0 | 38.7 | 1 | 38.7 |
CENLVK | 6 | 704.35 | 192.6 | 942.58 | 242.9 | 50.3 | 1 | 50.3 |
MACLLR | 6 | 705.37 | 203.8 | 943.59 | 248.2 | 44.4 | 1 | 44.4 |
HGCIVK | 6 | 655.35 | 204.8 | 893.57 | 249.2 | 44.4 | 1 | 44.4 |
FCFPLK | 6 | 753.39 | 219.9 | 991.61 | 262.4 | 42.5 | 1 | 42.5 |
VCHCLGK | 7 | 758.36 | 199.8 | 1234.81 | 295.0 | 95.2 | 2 | 47.6 |
DFCFPLK | 7 | 868.42 | 223.6 | 1106.64 | 267.0 | 43.4 | 1 | 43.4 |
MPLACLR | 7 | 802.42 | 223.6 | 1040.64 | 262.3 | 38.7 | 1 | 38.7 |
MTVCGGK | 7 | 694.31 | 182.2 | 1170.76 | 279.3 | 97.1 | 2 | 48.6 |
VGIHLCIK | 8 | 881.52 | 228.5 | 1119.75 | 275.2 | 46.7 | 1 | 46.7 |
MPALACLR | 8 | 873.46 | 228.3 | 1111.68 | 269.9 | 41.6 | 1 | 41.6 |
IPCCPVNLK | 9 | 985.51 | 232.3 | 1461.96 | 323.1 | 90.7 | 2 | 45.4 |
VIEVVQGACR | 10 | 1072.57 | 240.2 | 1310.80 | 290.5 | 50.3 | 1 | 50.3 |
IAFQELLCLR | 10 | 1204.66 | 271.6 | 1442.89 | 315.6 | 44.0 | 1 | 44.0 |
Average ΔΩ per Pal group: | 45.5 ± 4.1 |
Bold letters indicate palmitoylation sites.
Peptide masses are calculated from the molecular compositions.
Palmitoylated peptide masses are calculated from the molecular compositions. The number and location of the palmitoylation sites in the amino acid sequence are provided.
ΔΩ is the change in cross section after palmitoylation, defined as: ΔΩ = ΩPal peptide − Ωunmodified peptide