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. 2015 May 20;34(1):52. doi: 10.1186/s13046-015-0172-3

Table 1.

Primer sequences for the amplification of target genes

Genes Accession number Primer sequence (5′–3′) Product size (bp) Annealing temperature (°C)
VEGF NM_001025366 For: 5′-TTGCCTTGCTGCTCTACCTC-3′ 198 60
Rev: 5′-CACAGGATGGCTTGAAGATG-3′
MIP-2 NM_002089 For: 5′-AACCGAAGTCATAGCCACAC-3′ 150 59
Rev: 5′-CAGGAACAGCCACCAATAAG-3′
TGF-β1 NM_000660 For: 5′-CACGTGGAGCTGTACCAGAA-3′ 114 61
Rev: 5′-CACAACTCCGGTGACATCAA-3′
IL-6 NM_000600 For: 5′-GAGGAGACTTGCCTGGTGAA-3′ 267 60
Rev: 5′-GCGCAGAATGAGATGAGTTG-3′
IL-8 NM_000584 For: 5′-ACCGGAAGGAACCATCTCAC-3′ 822 61
Rev: 5′-GTGGATCCTGGCTAGCAGAC-3′
β-actin NM_001101 For: 5′-TGGACTTCGAGCAAGAGATG-3′ 207 60
Rev: 5′-GGATGTCCACGTCACACTTC-3′