Table 2.
Significant associations between tobacco smoking and methylation of CAD genes
| Current-Never | Adjusted model | Current-Former | Adjusted model | |||||
|---|---|---|---|---|---|---|---|---|
| CpG site | Estimate (se) | p-value | Estimate (se) | p-value | Estimate (se) | p-value | Estimate (se) | p-value |
| cg24908166 | −0.014 (0.003) | 1.1 × 10−7 | −0.015 (0.003) | 1.6 × 10−8 | −0.006 (0.002) | 0.02 | −0.007 (0.003) | 0.01 |
| cg12324353 | −0.012 (0.003) | 1.3 × 10−7 | −0.012 (0.002) | 3.5 × 10−7 | −0.007 (0.002) | 2.8 × 10−3 | −0.007 (0.002) | 2.1 × 10−3 |
| cg03725309 | −0.024 (0.005) | 2.0 × 10−7 | −0.022 (0.005) | 3.8 × 10−6 | −0.017 (0.004) | 4.2 × 10−5 | −0.016 (0.004) | 1.2 × 10−4 |
| cg00980784 | −0.015 (0.003) | 2.5 × 10−7 | −0.014 (0.003) | 3.5 × 10−6 | −0.015 (0.003) | 2.4 × 10−8 | −0.015 (0.003) | 8.6 × 10−8 |
| cg13916835 | −0.021 (0.004) | 3.0 × 10−7 | −0.021 (0.004) | 1.3 × 10−6 | −0.013 (0.004) | 1.5 × 10−3 | −0.013 (0.004) | 1.9 × 10−3 |
| cg09469355 | −0.017 (0.003) | 7.7 × 10−7 | −0.017 (0.003) | 8.5 × 10−7 | −0.015 (0.003) | 3.8 × 10−6 | −0.015 (0.003) | 1.6 × 10−6 |
| cg05603985 | −0.014 (0.003) | 8.4 × 10−7 | −0.013 (0.003) | 3.6 × 10−5 | −0.014 (0.003) | 1.3 × 10−7 | −0.014 (0.003) | 2.4 × 10−7 |
| cg04324276 | −0.015 (0.003) | 3.2 × 10−6 | −0.015 (0.003) | 1.2 × 10−5 | −0.010 (0.003) | 2.4 × 10−4 | −0.010 (0.003) | 5.2 × 10−4 |
| cg25468516 | −0.015 (0.003) | 3.6 × 10−6 | −0.014 (0.003) | 3.0 × 10−5 | 0.011 (0.003) | 2.3 × 10−4 | 0.012 (0.003) | 1.2 × 10−4 |
| cg22907952 | −0.011 (0.002) | 4.5 × 10−6 | −0.010 (0.003) | 6.9 × 10−5 | −0.007 (0.002) | 1.1 × 10−3 | −0.007 (0.002) | 1.5 × 10−3 |
| cg15474579 | −0.017 (0.004) | 5.0 × 10−6 | −0.013 (0.004) | 5.2 × 10−4 | −0.016 (0.003) | 2.0 × 10−6 | −0.016 (0.003) | 7.6 × 10−6 |
| cg20496896 | 0.012 (0.003) | 6.7 × 10−6 | 0.013 (0.003) | 4.6 × 10−6 | 0.005 (0.002) | 0.06 | 0.005 (0.002) | 0.03 |
| cg09397246 | 0.018 (0.004) | 1.0 × 10−5 | 0.018 (0.004) | 3.1 × 10−5 | 0.017 (0.003) | 5.6 × 10−7 | 0.017 (0.003) | 1.9 × 10−6 |
| cg26405020 | 0.014 (0.003) | 1.2 × 10−5 | 0.014 (0.003) | 1.4 × 10−5 | 0.008 (0.003) | 2.6 × 10−3 | 0.007 (0.003) | 8.2 × 10−3 |
| cg18236066 | −0.014 (0.003) | 1.3 × 10−5 | −0.015 (0.003) | 2.5 × 10−5 | −0.011 (0.003) | 1.4 × 10−4 | −0.011 (0.003) | 1.8 × 10−4 |
Bonferroni-corrected threshold 1.4 × 10−5
Current-Never: adjusted for age, sex, BMI, Houseman estimates, batch effects
Adjusted model: current-never, adjusted for age, sex, BMI, Houseman estimates, batch effects, systolic blood pressure, total cholesterol, triglycerides, daily alcohol intake, type 2 diabetes mellitus
Current-Former: adjusted for age, sex, BMI, Houseman estimates, batch effects
Adjusted model: current-former, adjusted for age, sex, BMI, Houseman estimates, batch effects, systolic blood pressure, total cholesterol, triglycerides, daily alcohol intake, type 2 diabetes mellitus