Table 1.
Specific primers used for general and real-time PCR.
| Primer | Primer sequences (5′-3′) | Temperature (°C) | Amplicon length (bp) | qPCR quantification target gene and function |
|---|---|---|---|---|
| Lf(YSK)-F Lf(YSK)-R |
GAAAACACTTGAGGACGG CGGATAAAACGGTTGATT |
56 56 |
168 | gyrB gene of L. ferriphilum YSK |
|
| ||||
| At(A01)-F At(A01)-R |
GACCCGTACCCTCAATCA CGGTTTCACTTCACTGGA |
58 58 |
175 | gyrB gene of At. thiooxidans A01 |
|
| ||||
| Lf-ArsB-F Lf-ArsB-R |
GGAGGCAAGGTCTGCGAGAA GGGAAAAGGGCGGCTCATTC |
62 65 |
138 | arsB gene of L. ferriphilum YSK, membrane-associated arsenite export pump |
|
| ||||
| Lf-ArsU-F Lf-ArsU-R |
GCCAATCCGGTCCTGGTTCA CCCATTTTCTTTTCGGGCTTTC |
65 64 |
146 | arsU gene of L. ferriphilum YSK, arsenic metabolism related protein of unknown function |
|
| ||||
| Lf-ArsRC-F Lf-ArsRC-R |
GAACGACAGGCATTTTGAGT GACGAGGACCTTTCTGACCA |
55 57 |
138 | arsRC gene of L. ferriphilum YSK, arsenate reductase/phosphotyrosine protein phosphatase gene |
|
| ||||
| At-ArsB-F At-ArsB-R |
AAAAGTCGCTGTGGGTGAAA GTTGGGTGCTTGTATTGCTG |
58 56 |
112 | arsB gene of At. thiooxidans A01, arsenical pump membrane protein |
|
| ||||
| At-ArsC-F At-ArsC-R |
ATGGCGGGTCCGAGGTAGGC GAGGGGTTTGCCACGGAAGG |
67 66 |
128 | arsC gene of At. thiooxidans A01, arsenate reductase |
|
| ||||
| At-ArsH-F At-ArsH-R |
TCCGACCATTCTACCAGTTCC AGCGGCTATTACGCCATTTT |
58 59 |
127 | arsH gene of At. thiooxidans A01, arsenical resistance protein |
|
| ||||
| At-ArsR-F At-ArsR-R |
ACTAATGCCCGAACTACGCT TATTGCCGAACATCTGGACC |
58 56 |
141 | arsR gene of At. thiooxidans A01, transcriptional regulator, a negative regulator of the ars operon |