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. 2015 May 26;6:109. doi: 10.3389/fneur.2015.00109

Figure 2.

Figure 2

Reduced neuron estimates for locus coeruleus (LC) and orexinergic neurons in CSD. (A) Optical fractionator stereological bilateral count estimates on 1:3 series of LC sections/mouse for mice exposed to Ctl (black bar) or CSD (gray bar) conditions obtained after a 4-week recovery opportunity for both groups (n = 5/group), **p < 0.01. (B) A similar stereological approach was used on a 1:2 series of orexinergic neuron sections/mouse for mice exposed to Ctl (black bar) or CSD (gray bar) conditions obtained after a 4-week recovery opportunity for both groups (n = 5/group), **p < 0.01. (C) Upper panels, representative images from mid LC in a Ctl (left) mouse and CSD mouse (right), immunolabeled with tyrosine hydroxylase (TH) and substrate blue. All TH+ and TH− cells are stained with Giemsa to provide nuclear details for counts and to identify non-TH neurons in LC nuclei. Calibration bar, 100 μm. Lower panels, similarly obtained images of perifornicular orexinergic neurons in Ctl and CSD mice. Calib bar, 200 μm. Note in CSD mice, both LC and orexinergic remaining neuronal somata are significantly smaller.