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. Author manuscript; available in PMC: 2015 May 26.
Published in final edited form as: Circulation. 2015 Mar 4;131(14):1278–1290. doi: 10.1161/CIRCULATIONAHA.114.013303

Figure 2.

Figure 2

Characterization of TERMINATOR, ALIEN, and PUNISHER, 3 novel developmentally regulated lncRNAs. A, Representative images of subcellular localization of TERMINATOR in human embryonic stem (ES) cells, ALIEN in vascular progenitors, and PUNISHER in primary endothelial cells as determined by RNA in situ hybridization using specific locked nucleic acid (LNA) probes. A scrambled control LNA probe has been tested in all 3 different cell types with similar results (representative pictures on the left). B, Quantification of nuclear and cytosolic lncRNA foci (n ≥5). C, Left, RNA-Seq read density coverage along lncRNA loci. Right, Gene ontology functional enrichment analysis from all genes exhibiting expression profiles similar to TERMINATOR (top), ALIEN (middle), or PUNISHER (bottom). D, Network depicting correlated gene expression profiles of the uncharacterized lncRNAs and key developmental transcription factors. A Pearson correlation threshold of 0.85 was used to define edges in the network. Thick gray lines indicate higher correlation relative to black lines. Data are represented as mean±SD. Scale bars: 25 μm (A, top) and 5 μm (A, bottom). HUVEC indicates human umbilical vein endothelial cell.