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. Author manuscript; available in PMC: 2015 May 26.
Published in final edited form as: Circulation. 2015 Mar 4;131(14):1278–1290. doi: 10.1161/CIRCULATIONAHA.114.013303

Figure 4.

Figure 4

In vivo functional evaluation of conserved lncRNAs during zebrafish development by morpholino (MO)-mediated loss of function. A, Morpholinos were designed to block specific highly conserved regions or putative splice sites and were injected into cardiovascular reporter zebrafish embryos. B, Representative phenotypes observed for the different knockdowns during vascular (fli1:GFP) and cardiac (cmlc2:GFP) development at 48 hours postfertilization (hpf). C and D, Quantification of vascular (C) and cardiac (D) phenotypes obtained for the different morpholinos (n>70). E and F, Analysis of defects in blood vessel formation by quantification of tube number (E) and tube length (F; n >70). G, Heart function measured as heartbeats per minute for the different morphants (n >10). Data are represented as mean±SD. Scale bars, 5 μm (B). Wt indicates wild-type. *P<0.05.