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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Neurobiol Aging. 2014 Sep 28;36(2):982–992. doi: 10.1016/j.neurobiolaging.2014.09.016

Fig. 4.

Fig. 4

Age-dependent changes in glycogen storage in intact hippocampi and hippocampal slices. (A) Glycogen levels were comparable in intact hippocampus in the 4 age groups. (B) Glycogen content in hippocampal slices at different time points after slice preparation. After preparation slices were kept at room temperature during the first hour of recovery and then transferred to ACSF maintained at 32 ° C to allow further recovery of the glycogen stores. Data are mean ± SEM (n = 4), *p < 0.05, 12–20 months and >22 months versus 1–2 months. Two-way ANOVA followed by Tukey multiple comparisons test. (C) The utilization of glycogen stores is significantly enhanced during moderate glucose deprivation in aged hippocampal slices; comparing slices from 1 to 2, 3 to 6, 12 to 20, and >22 months rats after exposure to 2.5 mM glucose for 60 minutes and collected at different time. Data are the mean ± SEM values n =4. *p < 0.05; >22 months versus 1–2 months. Two-way ANOVA followed by Tukey multiple comparisons test. Abbreviations: ACSF, artificial cerebrospinal fluid; ANOVA, analysis of variance; SEM, standard error of the mean.