Table 3.

Analysis of Phase I cytokine gates

GATE	TYPE OF GATE	N	5/6 OR 6/6 RESPONSES DETECTED AND NO FALSE-POSITIVE RESPONSES (N =37)	5/6 OR 6/6 RESPONSES DETECTED AND FALSE- POSITIVE RESPONSES (N =44)	<5/6 POSITIVE RESPONSES (N =28)
CD4+ Cytokine+ Gate: Positive cells	Gate includes all cytokine-positive cells	71	34 (92%)	13 (30%)	24 (86%)
	Gate does not include cytokine low cells	33	3 (8%)	28 (64%)	2 (7%)
	Gate does not include cytokine high cells	2	0 (0%)	1 (2%)	1 (4%)
	Other	3	0 (0%)	2 (5%)	1 (4%)
CD4+ Cytokine+ Gate: Gate proximity	Gate does not include negative cells and includes all cytokine-positive cells (high and low positives)	42	20 (54%)	13 (30%)	9 (32%)
	Gate too far from the cytokine-negative population (>1 log from negative population)	33	3 (8%)	28 (64%)	2 (7%)
	Gate too close to the cytokine-negative population (includes cytokine-negative cells)	31	14 (38%)	1 (2%)	16 (57%)
	Other	3	0 (0%)	2 (5%)	1 (4%)
CD8+ Cytokine+ Gate: Positive cells	Gate includes all cytokine-positive cells	71	33 (89%)	15 (34%)	23 (82%)
	Gate does not include cytokine low cells	35	4 (11%)	28 (64%)	3 (11%)
	Gate does not include cytokine high cells	1	0 (0%)	0 (0%)	1 (4%)
	Other	2	0 (0%)	1 (2%)	1 (4%)
CD8+ Cytokine+ Gate: Gate proximity	Gate does not include negative cells and includes all cytokine-positive cells (high and low positives)	41	19 (51%)	13 (30%)	9 (32%)
	Gate too far from the cytokine-negative population (>1 log from negative population)	35	4 (11%)	29 (66%)	2 (7%)
	Gate too close to the cytokine-negative population (includes cytokine-negative cells)	31	14 (38%)	1 (2%)	16 (57%)
	Other	2	0 (0%)	1 (2%)	1 (4%)