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. Author manuscript; available in PMC: 2015 Oct 24.
Published in final edited form as: Neuroscience. 2014 Aug 27;279:65–76. doi: 10.1016/j.neuroscience.2014.08.018

Figure 1. Systemic diphtheria toxin reduces the number of TpOH-ir neurons in the brainstem of Pet1/DTR mice but not WT mice.

Figure 1

A: Schematic representation of the locations used to count the number of TpOH-ir neurons in the RMg, RPa ROb, and VLM using coordinates described in the text as distance from Bregma (B). Adapted from Paxinos & Franklin, 2001. B: TpOH-ir neurons in the RMg, ROb, RPa and VLM from WT and Pet1/DTR mice after 4 μg DTT. Black bars, 100 μm. C: Number of TpOH-ir neurons/25 μm thick slice in Pet1/DTR mice (red circles, n = 17) and WT mice (black circles, n = 13) as a function of dose of DTT from 2–36 μg. D: Summary of the number of TpOH-ir neurons from Pet1/DTR mice injected with DT (red bar; n = 17 compared with WT mice injected with DT (black bar; n = 13 and Pet1/DTR mice that did not receive any injection (empty bar; n = 5. ****, p < 0.0001). No differences were found between DT treated WT mice and untreated Pet1/DTR mice. (p > 0.05, 1 way ANOVA, Bonferroni’s multiple comparison test).