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. 2014 Sep 3;40(2):488–501. doi: 10.1038/npp.2014.198

Figure 1.

Figure 1

Scheme of experimental procedure. Two groups of mice were exposed to CUS or kept in the home cage as controls for 5 weeks. Then, mice were daily injected with vehicle and drugs, respectively, for 1 week (week 6). In week 7, mice were tested for anxiety- and depression-like behavior, and pain perception in the hot plate test. The Von Frey's filaments test was carried out from weeks 8 to 11 at the indicated time points (bottom). NGF was injected three times to induce long-lasting hyperalgesia. NGF administration was carried out on days 0 and 4, immediately after the Von Frey's filaments test was performed on these days and finally on day 23. On day 24, the last Von Frey's filaments test was carried out and afterwards, tissues were isolated for analysis of eCB levels. Exposure to CUS was constantly applied for the entire duration of the experimental procedure. CUS, chronic unpredictable stress; EPM, elevated plus maze; FST, forced swim test; LDT, light–dark test; NGF, nerve growth factor.