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. Author manuscript; available in PMC: 2015 May 26.
Published in final edited form as: J AIDS Clin Res. 2014 Jun 10;5(6):315. doi: 10.4172/2155-6113.1000315

Figure 1. Comparison of baseline gene expression in PBMCs.

Figure 1

PBMCs from three patient groups (HIV negative controls, HIV+ ART patients, and LTNPs) were treated with 6 μL of Lipofectamine 2000 (mock transfection) for 22 hours. Total RNA was extracted and subjected to qRT-PCR. Baseline gene expression levels of IFN-λ1 (A), IFN-β (B) and RANTES (C) were calculated by comparing the Ct values of the gene of interest with the Ct value of GAPDH and subtracting this from 40 (40-delta Ct).