Table 1. IC50 values of the DBME and standard compounds for DPPH, ROS and RNS scavenging and iron chelation assays.
Name of Assay | DBME | Standard | Values of Standard compounds |
---|---|---|---|
DPPH | 7.56 ± 0.23 | Ascorbic acid | 5.29 ± 0.28 |
Hydroxyl radical (OH•) scavenging | 157.81 ± 1.24 | Mannitol | 571.45 ± 20.12 |
Superoxide anion (O2 •−) scavenging | 22.86 ± 0.45 | Quercetin | 42.06 ± 1.35 |
Hypochlorous acid (HOCl) scavenging | 90.17 ± 1.64 | Ascorbic acid | 235.96 ± 5.75 |
Singlet oxygen (1O2) scavenging | 414.40 ± 5.88 | Lipoic acid | 46.16 ± 1.16 |
Hydrogen peroxide (H2O2) scavenging | 12.93 ± 0.63 | Sodium pyruvate | 3.24 ± 0.30 |
Nitric oxide radical (NO) scavenging | 55.09 ± 1.31 | Curcumin | 90.82 ± 4.75 |
Peroxynitrite (ONOO-) scavenging | 1.55 ± 0.48 | Gallic acid | 0.876 ± 0.57 |
Iron chelating activity | 40.90 ± 0.31 | EDTA | 1.27 ± 0.05 |
# IC50 values of all activities are determined in μg/ml except in hydrogen peroxide and peroxynitrite scavenging assays where values express in mg/ml. Data expressed as mean ± S.D (n = 6). EDTA represents Ethylenediamine tetraacetic acid.