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. 2015 May 26;10(5):e0127976. doi: 10.1371/journal.pone.0127976

Fig 3. FOXD3 silencing promoted proliferation and inhibited starvation-induced apoptosis in SW1080 cells.

Fig 3

A: Western blotting analysis of FOXD3 and cleaved caspase-3 expression in SW1080 cells. B: Cell proliferation assay showing significantly enhanced proliferation rate of FOXD3-silenced SW1080 cells in comparison with siNC-treated SW1080 cells (P<0.05) by MTT assay. C: Representative images of EdU assay in SW1080 cells. D: Statistical results from five independent EdU assays. E: Representative results of apoptosis assay by flow cytometry in serum-starvated SW1080 cells. F: Statistical results from three independent apoptosis assays. Summarized results are expressed as mean ±SD. EV, empy vector; NC, negative control. Scale bar, 50μm.