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. 2015 May 26;10(5):e0127976. doi: 10.1371/journal.pone.0127976

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© 2015 Du et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — A: Western blotting analysis of FOXD3 and cleaved caspase-3 expression in U87MG cells. B: Cell proliferation assay showing significantly enhanced proliferation rate of FOXD3-overexpressed U87MG cells in comparison with EV-treated U87MG cells (P<0.05) by MTT assay. C: Representative images of EdU assay in U87MG cells. D: Statistical results from five independent EdU assays. E: Representative results of apoptosis assay by flow cytometry in serum-starvated U87MG cells. F: Statistical results from three independent apoptosis assays. Summarized results are expressed as mean ±SD. EV, empy vector; NC, negative control. Scale bar, 50μm.