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. 2015 May 26;10(5):e0127910. doi: 10.1371/journal.pone.0127910

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© 2015 Li et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 9 — F-actin organization was visualized using TRITC-conjugated phalloidin. Representative images of actin cytoskeleton structures in (A) DAOY-control-#1, (B) DAOY-control-#3, (C) DAOY-CRMP1-#5, (D) DAOY-CRMP1-#8, (E) ONS-76-control#1, (F) ONS-76-control#5, (G) ONS-76-CRMP1#4, (H) ONS-76-CRMP1#6, (I) UW228-1-control-#2, (J) UW228-1-control-#5, (K) UW228-1-CRMP1-#4, and (L) UW228-1-CRMP1-#6. Arrowheads represent filopodia and arrows indicate intense stress fibers. (M) The percentage of cells with filopodial protrusions was decreased in MB clones stably expressing CRMP1. A minimum of 100 individual cells of each clone were counted, and cells showing more than 20 filopodia were considered positive. (N) A minimum of 100 individual cells of each clone were counted to determine the percentage of cells bearing stress fibers in cell body. Data show the mean±SD from three individual experiments.