Table 2. Efficiency of transcription and translation of phoA constructs with a GTG (TGP) or ATG (TAP) translational start codon in M. gallisepticum transformants.
Transformant | Alkaline phosphatase (mean units/mg ± SEM) | mRNA (mean ± SEM) | Relative efficiency of translation (mean ± SEM) |
---|---|---|---|
TGP2 | 118.7 ± 11.3 | 4.8 ± 0.8 | 27.5 ± 8.5 |
TGP3 | 142.4 ± 18.2 | 6.4 ± 1.1 | 23.8 ± 4.8 |
TGP5 | 94.5 ± 3.9 | 3.5 ± 0.5 | 28.4 ± 5.7 |
TAP3 | 259.4 ± 5.9 | 12.5 ± 1.5 | 21.4 ± 2.9 |
TAP4 | 172.2 ± 7.8 | 10.9 ± 1.4 | 16.3 ± 1.9 |
TAP9 | 160.5 ± 1.2 | 13.4 ± 1.5 | 12.3 ± 1.4 |