Table 1. Development of early embryos after siRNA targeting Dnmt1 injection into GV stage oocytes.
| Pattern | Oocyte injection | No. embryos (rep) ※ | No. embryos cleaved (%±SEM) | No. blastocysts (%±SEM) | Blastocyst cell numbers (mean± SEM) & |
|---|---|---|---|---|---|
| PA | control | 151(3) | 141 (93.36±1.83) a | 55 (36.49±1.55) a | 37±3 (n = 55) |
| water | 144(3) | 131 (91.08±1.50) a b | 52 (35.99±1.72) a | 36±2 (n = 51) | |
| negative | 145(3) | 134 (92.46±1.76) a | 51 (35.06±2.32) a | 37±4 (n = 51) | |
| siRNA | 154(3) | 133 (86.39±0.90) b | 28 (18.22±1.87) b | 35±2 (n = 26) | |
| IVF | control | 140(3) | 100 (71.19±2.54) a | 26 (18.56±0.40) a | 38±2 (n = 25) |
| water | 141(3) | 99 (70.42±1.34) a | 26 (18.72±1.89) a | 37±3 (n = 26) | |
| negative | 141(3) | 99 (70.18±1.14) a | 25 (17.83±1.35) a | 37±2 (n = 25) | |
| siRNA | 159(3) | 103 (64.84±1.17) b | 16 (10.35±1.65) b | 36±3 (n = 16) | |
| SCNT | control | 150(3) (68.06±2.46) a | 132 (88.09±1.59) a | 30 (20.20±1.64) a | 36±3 (n = 29) |
| water | 149(3) (66.22±1.60) a b | 131 (87.81±2.25) a | 27 (18.13±1.16) a | 35±2 (n = 27) | |
| negative | 149(3) (66.39±2.52) a b | 132 (88.55±1.85) a | 29 (19.44±1.09) a | 37±3 (n = 28) | |
| siRNA | 157(3) (60.32±1.02) b | 126 (80.21±1.56) b | 19 (12.06±1.38) b | 35±3 (n = 18) |
After siRNA injection into GV stage oocytes, the matured oocytes were subjected to PA, IVF and SCNT, then the cleavage (at 48 h) and blastocyst (at 156 h) rates and blastocyst cell numbers of PA, IVF and SCNT embryos were examined, and siRNA injection impaired the development of PA, IVF and SCNT embryos.
※Embryos in the NT groups were the fused embryos, and the percentages in the bracket were the fusion rates.
&Blastocyst cell numbers of less than 16 were not included.
a-bValues in the same column with different superscripts differed significantly (P<0.05).