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. Author manuscript; available in PMC: 2015 May 26.
Published in final edited form as: J Peripher Nerv Syst. 2011 Dec;16(4):277–286. doi: 10.1111/j.1529-8027.2011.00362.x

Figure 3.

Figure 3

Biotinylated tumor necrosis factor-alpha (TNF-α) detected using neutravidin-fluorescein isothiocyanate (FITC) (green) in the spinal cord dorsal horn ipsilateral to sciatic CCI nerve injury 96 h after intra-sciatic injection. Sections were dual-labeled with neuronal marker NeuN (A) for large neurons, NF200 (B) for small neurons, calcitonin gene-related peptide (CGRP) (C) for CGRP+ fibers, Rip (D) for oligodendrocytes, glial fibrillary acidic protein (GFAP) (E) for astrocytes, or Ox42 (F) for microglia. TNF-α colocalized (yellow) with all markers except Ox42. Magnification 1200× (A, C, D) or 600× (B, E, F).